Technical Abstract: The National German Strawberry Genebank includes 369 cultivars and the active field collection in Dresden-Pillnitz also contains 318 Fragaria wild species accessions. Conservation of clonal crops requires safety duplication. An earlier calculation of the effort required to establish and maintain a safety duplication of the whole collection with tissue culture plants indicated that it is too labor intensive to be maintained. The development of an effective method for cryopreservation in liquid nitrogen would be more cost effective l. Four cryopreservation protocols were tested. All four techniques were equally effective for the strawberry cultivars tested, however the Fragaria species grew best with only one of the techniques. This technique will be the standard method for cryopreserving the strawberry collection. Twenty strawberry types are now in the permanent cryopreservation collection.