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United States Department of Agriculture

Agricultural Research Service

Research Project: FUNCTIONAL GENOMICS FOR IMPROVING NUTRIENTS AND QUALITY IN ALFALFA AND SOYBEAN

Location: Plant Science Research

Title: Phenotypic and genomic analysis of a fast neutron mutant population resource in soybean

Authors
item Bolon, Yung Tsi
item Haun, William -
item Xu, Wayne -
item Grant, David
item Gerhardt, Daniel -
item Albert, Thomas -
item Richmond, Todd -
item Jeddeloh, Jeffrey -
item Muehlbauer, Gary -
item Orf, James -
item Naeve, Seth -
item Stupar, Robert -
item Vance, Carroll

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 16, 2010
Publication Date: January 14, 2011
Citation: Bolon, Y.E., Haun, W.J., Xu, W., Grant, D.M., Gerhardt, D.J., Albert, T.J., Richmond, T., Jeddeloh, J.A., Muehlbauer, G., Orf, J., Naeve, S., Stupar, R.M., Vance, C.P. 2011. Phenotypic and genomic analysis of a fast neutron mutant population resource in soybean [abstract]. XIX Plant and Animal Genome Conference Proceedings, January 15-19, 2011, San Diego, California. Abstract No. P390. Available: http://www.intl-pag.org/19/abstracts/P05f_PAGXIX_390.html.

Technical Abstract: Mutagenized populations have become indispensable resources for introducing variation and studying gene function in plant genomics research. We utilized fast neutron radiation to induce deletion mutations in the soybean genome and phenotypically screened the resulting population. We exposed approximately 120,000 soybean seed to fast neutron radiation doses of up to 32 Gy and developed more than 20,000 independent M2 lines. Moreover, we demonstrate the utility of this population for phenotypic screening and associated genomic characterization of striking and agronomically important traits. We catalogued a database of plant variation in seed composition, maturity, morphology, pigmentation, and nodulation. Notably, we identified mutants that showed significant increases or decreases in seed protein and oil content across three generations and environments. A subset of the notable mutant lines were genotyped using comparative genome hybridization and exome resequencing technologies, revealing deleted genomic regions and candidate genes associated with phenotypes of interest. We present this fast neutron mutant soybean population as a valuable public resource for future genetic screens and functional genomics research.

Last Modified: 8/21/2014
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