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United States Department of Agriculture

Agricultural Research Service

Research Project: MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES

Location: Tick and Biting Fly Research

Title: Rhipicephalus microplus strain Deutsch, whole genome shotgun sequencing project Version 2

Authors
item Moolhuijzen, P -
item Guerrero, Felix
item Bellgard, M -

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: July 14, 2010
Publication Date: June 29, 2011
Citation: Moolhuijzen, P., Guerrero, F., Bellgard, M. 2011. Rhipicephalus microplus strain Deutsch, whole genome shotgun sequencing project Version 2. National Center for Biotechnology Information (NCBI).

Interpretive Summary: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. Cot filtration/selection techniques were used to reduce the repetitive fraction of the tick genome and enrich for the fraction of DNA with gene-containing regions. The first version of the genome sequencing of Cot-selected genomic DNA was sequenced using next generation 454 FLX pyrosequencing and assembled with an EST-based gene index of 14,587 unique entries serving as seed sequences. This second version consists of new data reassembled with the original data from the first version submission. The new data consists of 6 full plate runs of 454 Titanium pyrosequencing of Cot-selected genomic DNA. This Whole Genome Sequencing Project was given the Project accession ADMZ00000000. This version of the project (02) has the accession number ADMZ02000000, and this submission consists of 175,208 contig sequences with Accession Numbers ADMZ02000001-ADMZ02175208.

Technical Abstract: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. Cot filtration/selection techniques were used to reduce the repetitive fraction of the tick genome and enrich for the fraction of DNA with gene-containing regions. The first version of the genome sequencing of Cot-selected genomic DNA was sequenced using next generation 454 FLX pyrosequencing and assembled with an EST-based gene index of 14,587 unique entries serving as seed sequences. This second version consists of new data reassembled with the original data from the first version submission. The new data consists of 6 full plate runs of 454 Titanium pyrosequencing of Cot-selected genomic DNA. This Whole Genome Sequencing Project was given the Project accession ADMZ00000000. This version of the project (02) has the accession number ADMZ02000000, and this submission consists of 175,208 contig sequences with Accession Numbers ADMZ02000001-ADMZ02175208.

Last Modified: 10/1/2014
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