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United States Department of Agriculture

Agricultural Research Service

Research Project: MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES

Location: Tick and Biting Fly Research

Title: Rhipicephalus (Boophilus) microplus strain Deutsch, whole genome shotgun sequencing project first submission of genome sequence

Authors
item Inman, J -
item Caler, E -
item Guerrero, Felix

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: June 10, 2010
Publication Date: June 11, 2010
Citation: Inman, J.M., Caler, E., Guerrero, F. 2010. Rhipicephalus (Boophilus) microplus strain Deutsch, whole genome shotgun sequencing project first submission of genome sequence. National Center for Biotechnology Information (NCBI).

Interpretive Summary: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. Cot filtration/selection techniques were used to reduce the repetitive fraction of the tick genome and enrich for the fraction of DNA with gene-containing regions. The Cot-selected genomic DNA was sequenced using next generation 454 FLX pyrosequencing and assembled with an EST-based gene index of 14,587 unique entries serving as seed sequences. This submission of the Whole Genome Sequencing Project was given the Project accession ADMZ00000000. This version of the project (01) has the accession number ADMZ01000000, and this submission consists of 315,428 contig sequences with Accession Numbers ADMZ01000001-ADMZ01315428.

Technical Abstract: The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence difficult. Cot filtration/selection techniques were used to reduce the repetitive fraction of the tick genome and enrich for the fraction of DNA with gene-containing regions. The Cot-selected genomic DNA was sequenced using 454 FLX pyrosequencing and assembled with an EST-based gene index of 14,587 unique entries serving as seed sequences. This submission of the Whole Genome Sequencing Project was given the Project accession ADMZ00000000. This version of the project (01) has the accession number ADMZ01000000, and this submission consists of 315,428 contig sequences with Accession Numbers ADMZ01000001-ADMZ01315428.

Last Modified: 7/23/2014
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