Location: Poultry Microbiological Safety Research
Title: Prevalence of Salmonella Following Immersion Chilling for Matched Neck Skin, Whole Carcass Rinse, and Whole Carcass Enrichment Sampling Methodologies Authors
Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 2010
Publication Date: January 24, 2011
Citation: Buhr, R.J., Holmes, J.M., Cason Jr, J.A., Cox Jr, N.A., Bourassa, D.V., Rigsby, L.L., Cray, P.J. 2011. Prevalence of salmonella following immersion chilling for matched neck skin, whole carcass rinse, and whole carcass enrichment sampling methodologies. International Poultry Scientific Forum. 59, P.18 (ABST). Technical Abstract: Salmonella prevalence and the serogroups recovered following immersion chilling were determined for matched enriched neck skin, whole carcass rinse, and whole carcass samples. Commercially processed and eviscerated broiler carcasses were chilled in ice/tap water 40 min with or without 20 ppm free chlorine. From each carcass, the neck skin (8.3 g) was removed and stomached in 83 mL 1% buffered peptone water (BPW). Next the carcass was rinsed in 430 mL 1% BPW for 1 min and 30 mL of rinsate collected. The carcass was then subjected to whole carcass enrichment in the remaining 400 mL 1% BPW. All samples were incubated at 37C for 24 h before aliquots were transferred to selective enrichment broths (RV and TT). Following incubation, BGS and MLIA plates were streaked and incubated at 37C for 24 h. From each plate, three colonies displaying Salmonella characteristics were individually stabbed into TSI and LIA slants from which Salmonella serogroups were identified by antibody agglutination. Among the non-chlorinated carcasses for neck skin samples, 10/20 were Salmonella-positive and were serogroups B or C3. For whole carcass rinse, 10/20 were Salmonella-positive and were all C3. For whole carcass enrichment, all 20 were Salmonella-positive and were B, C2, C3, or E. Among the chlorinated carcasses for neck skin samples, 7/20 were Salmonella-positive and were serogroups B or C3. For whole carcass rinse no samples were Salmonella-positive. For whole carcass enrichment 19/20 were Salmonella-positive and were B or C3. Salmonella serogroups B and C3 were detected on 4/17 Salmonella-positive neck skin samples and in 17/19 Salmonella-positive whole carcass enrichment samples, but only two carcasses had both serogroups recovered in both the neck skin and the matching enriched carcass samples. In this study, whole carcass enrichment detected Salmonella on 100 and 95% (nonchlorinated and chlorinated chilled, respectively) of the samples, versus 50 and 35% of the neck skin samples, or 50 and 0% of the carcass rinse samples.