Technical Abstract: Potyviruses, transmitted by a diverse array of common aphid species, infect a broad range of vegetable crops, and can be problematic in glasshouse tomato production. Once introduced, these viruses are believed to be transmitted plant-to-plant during pruning operations, and can infect large sections of a greenhouse, resulting in significant losses in fruit quality and yield. A number of methods are often used for virus management in greenhouse production, including rouging of diseased plants and treatment of tools and facilities with virucides to potentially eradicate the virus responsible. In order to clarify potyvirus transmission efficiency from an infected source during pruning operations, experiments were conducted using direct and serial mechanical inoculation of Potato virus Y (PVY) using a scalpel dipped in a suspension of PVY-infected plant sap. These tests demonstrated that both serial and direct inoculation resulted in significant PVY transmission, but that transmission rates declined rapidly after the first few plants in serial transmission. Additional tests evaluated the efficiency two virucides, quaternary ammonium solution and sodium hypochlorite, for virus inactivation during pruning operations using a range of concentrations and time points. Results demonstrated that 0.5% sodium hypochlorite treatment for as little as two seconds was sufficient for virus control, and clearly superior to treatment with quaternary ammonium solutions.