Technical Abstract: Recently, the channel catfish, Ictalurus puntatus' X blue catfish, I.furcatus ' F1 hybrid has demonstrated good potential for commercial production compared to the commonly raised channel catfish. Efforts by researchers and commercial producers in the last 40 years have improved hybrid fry production; however little research has been conducted related to improving sperm characteristics of the blue catfish, the male parent, blue catfish. Body weight, length, stage of maturation, gonadosomatic index, condition factor and blood plasma levels of testosterone in 114 individual mature blue catfish of 5 to 8 years in age were examined as predictors of sperm production. Sperm production in blue catfish exhibited a linear relationship with the stage of gonadal maturity and plasma testosterone (y=-5.776+2.51*stage of maturity – 0.01*testosterone, r2=0.39, p<0.03). Other variables measured were not related to sperm concentration (p>0.05). Improvements in prediction accuracy are needed to make this regression model a useful tool for predicting sperm production in blue catfish to improve the efficiency of hybrid catfish production.