INTEGRATED STRATEGIES FOR ADVANCE MANAGEMENT OF FRUIT, NUT, AND OAK TREE DISEASES
Location: Crops Pathology and Genetics Research
Title: Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains
Submitted to: Journal of General Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 16, 2012
Publication Date: February 15, 2012
Citation: Yakabe, L.E., Maccree, M.M., Sudarshana, P., Mcclean, A.E., Parker, S.R., Wechter, W.P., Presting, G., Kluepfel, D.A. 2012. Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains. Journal of General Plant Pathology. DOI: 10.1007/s10327-012-0364-z.
Novel primers were developed to amplify a 243 bp fragment of an intergenic region between gene5 and tms2 on the T-DNA of Agrobacterium tumefaciens. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence and did not generate extraneous bands, facilitating robust real-time PCR detection.