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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #259866
Title: Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains

Author
item Yakabe, Lani
item MACCREE, M - University Of California
item SUDARSHANA, P - Monsanto Corporation
item McClean, Ali
item PARKER, S - US Department Of Agriculture (USDA)
item Wechter, William - Pat
item PRESTING, G - University Of Hawaii
item Kluepfel, Daniel

Submitted to: Journal of General Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/16/2012
Publication Date: 2/15/2012
Citation: Yakabe, L.E., Maccree, M.M., Sudarshana, P., Mcclean, A.E., Parker, S.R., Wechter, W.P., Presting, G., Kluepfel, D.A. 2012. Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains. Journal of General Plant Pathology. DOI: 10.1007/s10327-012-0364-z.

Interpretive Summary:

Technical Abstract: Novel primers were developed to amplify a 243 bp fragment of an intergenic region between gene5 and tms2 on the T-DNA of Agrobacterium tumefaciens. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence and did not generate extraneous bands, facilitating robust real-time PCR detection.