Technical Abstract: Bacterial fruit blotch (BFB) caused by the bacterium Acidovorax avenae subsp. citrulli (Aac) is a seed-borne disease that threatens most cucurbit crops. Although, limited resistance has been found in a small number of Plant Introductions (PI) in watermelon (Citrullus spp.), no significant activity in screening melon (Cucumis spp.) PIs for resistance to this disease has been conducted. In this study, 332 Cucumis spp. PI were screened for resistance to Aac using a newly developed seed vacuum- infusion assay. Significant differences in the reaction of the PI to BFB were observed. The majority of lines were found to be extremely susceptible to the disease. However, several PI with lower levels of resistance were also identified. Variability in the reaction of plants within each PI was also observed. Of the 332 PI tested, 16 were selected for standard spray inoculation tests. PI 353814, PI 381171, PI 536573 and PI 614401 all belonging to C. melo and PI 504558 (C. ficifolius), were found to have significantly greater levels of resistance than susceptible control cultivars or other PIs in two independent spray inoculation tests. These PI may serve as potential sources of resistance to BFB in melon breeding programs.