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United States Department of Agriculture

Agricultural Research Service

Research Project: RANGELAND AND LIVESTOCK RESOURCE MANAGEMENT Title: Bovine adipose triglyceride lipase is not altered and adipocyte fatty acid binding protein is increased by dietary flaxseed

Authors
item Deiuliis, Jeffrey -
item Shin, Jonghyun -
item Murphy, Eric -
item Kronberg, Scott
item Eastridge, Maurice -
item Suh, Yeunsu -
item Yoon, Jong-Taek -
item Lee, Kichoon -

Submitted to: Lipids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 14, 2010
Publication Date: October 15, 2010
Citation: Deiuliis, J., Shin, J., Murphy, E., Kronberg, S.L., Eastridge, M.L., Suh, Y., Yoon, J., Lee, K. 2010. Bovine adipose triglyceride lipase is not altered and adipocyte fatty acid binding protein is increased by dietary flaxseed. Lipids. 45:963-973. DOI: 10.1007/s11745-010-3476-2.

Interpretive Summary: In this paper, we report the full length coding sequence of bovine ATGL cDNA are reported and analyze its expression in bovine tissues. Similar to human, mouse, and pig ATGL sequences, bovine ATGL has a highly conserved patatin domain that is necessary for lipolytic function in mice and humans. This suggests that ATGL is functionally intact as a triglyceride lipase enzyme in cattle. Tissue distribution of ATGL gene expression was highest in fat and muscle (skeletal and cardiac) tissue, while protein expression was solely detectible in the adipose tissue. The effect of 109 days of flaxseed supplementation on ATGL and adipocyte fatty acid binding protein (FABP4 or A-FABP, E-FABP or FABP5) expression was examined in Angus steers. Supplemented steers had greater triacylglycerol content in the muscle compared with unsupplemented steers. Additionally, supplementation increased A-FABP expression and decreased stearoyl-CoA desaturase 1 (SCD-1) expression in muscle, while total ATGL expression was unaffected. In summary, supplementation of cattle rations with flaxseed increased muscle triacylglycerol concentrations; attributed in part to increased expression of key enzymes involved in lipid trafficking (A-FABP) and metabolism (SCD-1).

Technical Abstract: In this paper, we report the full length coding sequence of bovine ATGL cDNA are reported and analyze its expression in bovine tissues. Similar to human, mouse, and pig ATGL sequences, bovine ATGL has a highly conserved patatin domain that is necessary for lipolytic function in mice and humans. This suggests that ATGL is functionally intact as a triglyceride lipase in cattle. Tissue distribution of ATGL gene expression was highest in fat and muscle (skeletal and cardiac) tissue, while protein expression was solely detectible in the adipose tissue. The effect of 109 days of flaxseed supplementation on ATGL and adipocyte fatty acid binding protein (FABP4 or A-FABP, E-FABP or FABP5) expression was examined in Angus steers. Supplemented steers had greater triacylglycerol content in the muscle compared with unsupplemented. Additionally, supplementation increased A-FABP expression and decreased stearoyl-CoA desaturase 1 (SCD-1) expression in muscle, while total ATGL expression was unaffected. In summary, supplementation of cattle rations with flaxseed increased muscle TAG concentrations; attributed in part to increased expression of key enzymes involved in lipid trafficking (A-FABP) and metabolism (SCD-1).

Last Modified: 12/18/2014
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