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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT AND TREATMENT OF DRAINAGE WATERS FOR WATER QUALITY PROTECTION AND SUSTAINABILITY OF AGRICULTURAL PRODUCTION IN THE MIDWEST U.S.

Location: Soil Drainage Research

Title: Expression of Root-Related Transcription Factors Associated with Flooding Tolerance of Soybean

Authors
item Vantoai, Tara
item Alves, Jose -
item Valliyodan, Babu -
item Goulart, Patricia -
item Lee, Jeong -
item Fritschi, Felix -
item Rahman, Mohammed -
item Shannon, Grover -

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2010
Publication Date: August 8, 2010
Citation: Vantoai, T.T., Alves, J., Valliyodan, B., Goulart, P. 2010. Expression of Root-Related Transcription Factors Associated with Flooding Tolerance of Soybean. [abstract]. Biennial Conference on Molecular and Cellular Biology of the Soybean.

Technical Abstract: Much flooding research focuses on gene expression changes of the model plant Arabidopsis in response to low-oxygen stress. However, ample evidence indicates that flooding tolerance involves more than low-oxygen tolerance. This study addressed the physiological response and differential expression of root-related transcription factors (TFs) associated with tolerance of soybean to soil flooding. We evaluated the differential soil flooding responses of PI408105A and S99-2281 plants at the physiological, morphological and anatomical levels, and used qRT-PCR to identify the underlying gene expressions. Flood-sensitive S99-2281 root biomasses remained unchanged during the ten-day flooding. Flood-tolerant PI408105A plants exhibited root growth recovery after three days of flooding and growth accelerated between days 7 and 10. Flooding-induced aerenchyma and adventitious roots developed faster in PI408105A than in S99-2281 genotype. Quantitative transcript analysis identified four potentially flood-tolerant genes that differentially expressed between the two genotypes during the entire duration. Three of these four genes were TFs; the fourth was Hemoglobin GLB1. The differential gene expression associated with flooding tolerance was quantitative and temporal. Further analyses of these genes will identify their potential to enhance flooding tolerance of soybean cultivars.

Last Modified: 10/1/2014
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