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United States Department of Agriculture

Agricultural Research Service

Research Project: SIV EPITOPES AND GENETIC FACTORS THAT AFFECT SURVEILLANCE AND IMMUNITY ELICITED BY INFLUENZA VACCINES AGAINST EMERGING INFLUENZA STRAINS Title: Comparison of Humoral and Cellular Immune Responses to Inactivated Swine Influenza Virus Vaccine in Weaned Pigs

Authors
item Platt, Ratree -
item Gauger, Phillip -
item Kimura, Kayoko -
item Roth, James -
item VINCENT, AMY
item LOVING, CRYSTAL
item Zanella, Eraldo -
item LAGER, KELLY
item Kehrli Jr, Marcus

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 30, 2010
Publication Date: December 7, 2010
Citation: Platt, R., Gauger, P., Kimura, K., Roth, J., Vincent, A., Loving, C., Zanella, E., Lager, K., Kehrli, M. 2010. Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 085P/85P.

Technical Abstract: Purpose: To evaluate and compare humoral and cellular immune responses to inactivated swine influenza virus (SIV) vaccine. Methods: Fifty 3-week-old weaned pigs from a herd free of SIV and PRRSV were randomly divided into the non-vaccinated control group and vaccinated group containing 25 pigs each. Pigs were vaccinated intramuscularly twice with adjuvanted UV-inactivated A/SW/MN/02011/08 (MN/08) H1N1 SIV vaccine at 6 and 9 weeks of age. Whole blood samples for multi-parameter flow cytometry (MP-FCM) and serum samples for hemagglutination inhibition (HI) assay were collected at 23 and 28 days after the second vaccination respectively. A standard HI assay and MP-FCM were performed against UV-inactivated homologous MN/08 and heterologous pandemic A/CA/04/2009 H1N1 (CA/09) viruses. Results: While the HI assay detected humoral responses only to the MN/08 virus, the MP-FCM detected strong cellular responses against the MN/08 delta-cluster virus and lower but significant heterologous responses to the CA/09 gamma-cluster virus, especially in the CD4+CD8+ T cell subset. Conclusions: The cellular heterologous responses to UV-inactivated gamma-cluster virus by MP-FCM suggested that the assay was sensitive and potentially detected a wider range of antigens than what is detected by the HI assay. The MP-FCM also identified the responses of multiple parameters in different T cell subsets. Overall, the adjuvanted UV-inactivated A/SW/MN/02011/08 H1N1 SIV vaccine stimulated both humoral and cellular immune responses including the CD4-CD8+ T cell subset.

Last Modified: 8/27/2014
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