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United States Department of Agriculture

Agricultural Research Service

Research Project: FOOD FACTORS AND MAINTENANCE OF BODY WEIGHT AND HEALTH Title: Nadh Fluorescence Lifetime Analysis of the Effect of Magnesium Ions on Aldh2

Authors
item Gonnella, Thomas -
item Leedahl, Travis -
item PICKLO, MATTHEW

Submitted to: Chemico Biological Interactions
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 20, 2011
Publication Date: May 30, 2011
Repository URL: http://handle.nal.usda.gov/10113/58219
Citation: Gonnella, T.P., Leedahl, T.S., Picklo, M.J. 2011. NADH fluorescence lifetime analysis of the effect of magnesium ions on ALDH2. Chemico Biological Interactions. 191(1-3):147-152.

Interpretive Summary: Aldehyde dehydrogenase (ALDH) enzymes play key roles in the detoxification of aldehydes generated from oxidative stress. The activity of ALDH enzymes is altered by changes in magnesium ion concentration by altering the binding of the NADH product of enzyme activity. In this work we applied a new technique, fluorescence lifetime analysis, to the study of ALDH activity and how it is influenced by magnesium ions. Our data indicate that magnesium regulates ALDH activity through changing the binding characteristics of NADH and that the ALDH-NADH complex can be present in multiple states during enzyme activity. These data provide greater insight into the physiologic role of magnesium in regulating enzyme activities in the cell.

Technical Abstract: Aldehyde dehydrogenase 2 (ALDH2) catalyzes oxidation of toxic aldehydes to carboxylic acids. Physiologic levels of Mg2+ ions influence enzyme activity in part by increasing NADH binding affinity. Traditional fluorescence measurements monitor the blue shift of the NADH fluorescence spectrum to study ALDH2-NADH interactions. By using time-resolved fluorescence spectroscopy, we have resolved free NADH (t = 0.4 ns) and bound NADH (t = 6.0 ns). We used this technique to investigate the effects of Mg2+ on the ALDH2-NADH binding characteristics and enzyme catalysis. From the resolved free and bound NADH fluorescence signatures, the KD for NADH with ALDH2 ranged from 334 µM to 10 µM for Mg2+ ion concentrations of 2 µM to 6000 µM, respectively The dissociation rate of NADH ranged from 0.4 s-1 (6000 µM Mg2+) to 8.3 s-1 (0 µM Mg2+) by by displacing NADH from ALDH2 with the addition of excess NAD+ and resolving the real-time NADH fluorescence signal. The calculated NADH association/re-association rates were approximately 0.04 s-1 over the entire Mg2+ ion concentration range and demonstrate that Mg 2+ ions slow the release of NADH from the enzyme rather than promoting its re-association. We applied NADH fluorescence lifetime analysis to the study of NADH binding during enzyme catalysis. Our fluorescence lifetime analysis confirmed the biphasic behavior of the enzyme activity as a function of Mg2+ concentration. Importantly, we observed no pre-steady state burst of NADH formation in the absence of Mg2+, and our results indicate that previously reported burst activity in the absence of metal may be due to trace levels of metal present in these assays. Furthermore, we observed distinct fluorescence signatures from multiple ALDH2-NADH complexes corresponding to free NADH, enzyme-bound NADH, and, potentially, an abortive NADH-enzyme-propanal complex (t = 11.2 ns).

Last Modified: 9/10/2014
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