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Research Project:
MANAGEMENT OF GENETIC RESOURCES FOR VITIS, PRUNUS, JUGLANS, FICUS, OLEA, PISTACIA, PUNICA, DIOSPYROS, ACTINIDIA, AND MORUS
Location: National Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes
Title: Efficiencies in alginate encapsulation of vegetative explants
Authors
Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 1, 2010
Publication Date: August 17, 2010
Repository URL:
http://www.ars.usda.gov/main/site_main.htm?modecode=53-06-20-00
Citation: George, L.J., Preece, J.E. 2010. Efficiencies in Alginate Encapsulation of Vegetative Explants. HortScience. 45(8):S163
Interpretive Summary: The goal of this study was to improve a non-mechanized bulk
encapsulation technique to standardize encapsulation procedures
and reduce the labor time compared to encapsulating individual
nodes. Four mm-long nodal segments from Stage II cultures of
Hibiscus moscheutos L. ‘Lord Baltimore’ were encapsulated as
groups of 5 segments in matrix masses gelled with 2.5 - 3.25%
sodium alginate that were solidified with 60 - 90mM calcium
chloride in various experiments. Encapsulated masses were placed
in sterile Petri dishes, sealed with parafilm, and placed in
darkness at 5 °C for four weeks. They were then removed from
refrigeration and placed on fresh Stage II medium and incubated
at 25 °C under cool white fluorescent lamps for four weeks when
data were taken. There was a significant interaction between the
alginate viscosity and the calcium chloride concentration for
subsequent shoot length. The most axillary shoots grew when
the masses were encapsulated in 3% alginate and 60 mM CaCl2.
The longest shoots grew from nodal segments encapsulated with
2.75% alginate that was solidified with 60 mM CaCl2. The most
roots grew from masses of nodal segments encapsulated in 2.5%
alginate solidified with 60 mM CaCl2.
Technical Abstract:
The goal of this study was to improve a non-mechanized bulk
encapsulation technique to standardize encapsulation procedures
and reduce the labor time compared to encapsulating individual
nodes. Four mm-long nodal segments from Stage II cultures of
Hibiscus moscheutos L. ‘Lord Baltimore’ were encapsulated as
groups of 5 segments in matrix masses gelled with 2.5 - 3.25%
sodium alginate that were solidified with 60 - 90mM calcium
chloride in various experiments. Encapsulated masses were placed
in sterile Petri dishes, sealed with parafilm, and placed in
darkness at 5 °C for four weeks. They were then removed from
refrigeration and placed on fresh Stage II medium and incubated
at 25 °C under cool white fluorescent lamps for four weeks when
data were taken. There was a significant interaction between the
alginate viscosity and the calcium chloride concentration for
subsequent shoot length. The most axillary shoots grew when
the masses were encapsulated in 3% alginate and 60 mM CaCl2.
The longest shoots grew from nodal segments encapsulated with
2.75% alginate that was solidified with 60 mM CaCl2. The most
roots grew from masses of nodal segments encapsulated in 2.5%
alginate solidified with 60 mM CaCl2.
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Last Modified: 05/21/2013
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