MOLECULAR CHARACTERIZATION AND GASTROINTESTINAL TRACT ECOLOGY OF COMMENSAL HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN THE CHICKEN
Location: Poultry Microbiological Safety Research
Title: The molecular-genetic analysis of Clostridium perfringens strains isolated from broilers on farms in Central Russia
| Volozhantsev, Nikolay - |
| Bannov, Vasily - |
| Krasilnikova, Valentina - |
| Kudryavtseva, Tamara - |
| Myakinina, Vera - |
| Svetoch, Eduard - |
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 2010
Publication Date: September 22, 2010
Citation: Seal, B.S., Volozhantsev, N.V., Bannov, V.A., Krasilnikova, V.M., Kudryavtseva, T., Myakinina, V.P., Svetoch, E.A. 2010. The molecular-genetic analysis of Clostridium perfringens strains isolated from broilers on farms in Central Russia. Meeting Abstract.
The objective of the research was to perform phenotypic and molecular-genetic typing of Clostridium perfringens strains commonly spread on poultry farms in Central Russia. Samples of homogenized iliac and cecal contents from 760 broilers were assayed and 325 C. perfringens strains (42.8 %) were isolated. The strains were genetically identified by ribosomal genes, as well as by genes cpa, cpb, cpb2 and cpe responsible for the primary toxin production. All the bacterial isolates were found to carry the a-toxin gene cpa. The ß-toxin gene cpb was identified in 23 % of the strains, cpb2 in 72 %, and cpb and cpb2 were concurrently found in 13 % of cases. The gene cpe producing enterotoxin as a main cause of human food poisoning was not found in any of the examined strains. Plasmid DNA was present in 66 % of the strains and was correlative either to the gene cpb or to cpb2 in most cases. The RAPD-PCR method allowed for comparative typing of the isolated C. perfringens strains and those provided by the R.B. Russell Agricultural Research Center (ARS/USDA, USA.). All these strains formed four primary clusters. Cluster 1 comprised Russian strains only, not carrying the gene cpb2 for the most part. Over 90 % of the American strains formed clusters 2 and 3, and strains isolated from necrotic enteritis birds had similar RAPD profiles in spite of being different in the gene cpb and plasmid DNA and formed an individual cluster. Strains of several C. perfringens genotypes were generally identified within a single farm, whereas strains of the same genotype could be identified in different regions.