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United States Department of Agriculture

Agricultural Research Service

Research Project: RESPONSE OF DIVERSE RICE GERMPLASM TO BIOTIC AND ABIOTIC STRESSES

Location: Dale Bumpers National Rice Research Center

Title: Mapping Quantitative Trait Loci for Resistance to Rice Blast

Authors
item Jia, Yulin
item Liu, Guangjie -

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 20, 2010
Publication Date: February 1, 2011
Citation: Jia, Y., Liu, G. 2011. Mapping quantitative trait loci for resistance to rice blast. Phytopathology. 101(2):176-181.

Interpretive Summary: Quantitative trait loci (QTLs) of rice conferring resistance to rice blast, caused by Magnaporthe oryzae, have been under-explored. In the present study, we mapped blast resistant QTLs using composite interval mapping with the six races IB1, IB45, IB49, IB54, IC17, and ID1 of M. oryzae using a recombinant inbred line (RIL) population derived from a cross of the moderately- susceptible japonica cultivar Lemont with the moderately- resistant indica cultivar Jasmine 85. Disease reactions of 227 RILs were evaluated using a category scale of ratings from 0, representing the most resistant, to 5, representing the most susceptible. A total of eight QTLs responsive to different degrees of phenotypical variation ranging from 5.2 to 26.5% were identified on chromosomes 3, 8, 9, 11, and 12. This study demonstrates the usefulness of studying blast QTLs using physiological races by multiple interval mapping under greenhouse conditions.

Technical Abstract: Quantitative trait loci (QTLs) conferring resistance to rice blast, caused by Magnaporthe oryzae, have been under-explored. In the present study, composite interval mapping was used to identify the QTLs that condition resistance to the six out of the twelve common races IB1, IB45, IB49, IB54, IC17, and ID1 of M. oryzae using a recombinant inbred line (RIL) population derived from a cross of the moderately- susceptible japonica cultivar Lemont with the moderately-resistant indica cultivar Jasmine 85. Disease reactions of 227 F7 RILs were determined using a category scale of ratings from 0, representing the most resistant, to 5, representing the most susceptible. A total of nine QTLs responsive to different degrees of phenotypic variation ranging from 5.17 to 26.53% were mapped on chromosomes 3, 8, 9, 11, and 12: qBLAST3 at1.9 centimorgen (cM) to simple sequence repeat (SSR) marker RM282 on chromosome 3 to IB45 accounting for 5.17%; qBLAST8.1 co-segregated with SSR marker RM1148 to IB49 accounting for 6.69%, qBLAST8.2 at 0.1 cM to SSR marker RM72 to IC17 on chromosome 8 accounting for 7.22%; qBLAST9.1 at 0.1 cM to SSR marker RM257 to IB54, qBLAST9.2 at 2.1 cM to SSR marker RM108, and qBLAST 9.3 at 0.1 cM to SSR marker RM215 to IC17 on chromosome 9 accounting for 4.64%, 7.62% and 4.49 %; qBLAST11 at 2.2 cM to SSR marker RM244 to IB45 and IB54 on chromosome 11 accounting for 26.53% and 19.60% ; qBLAST12.1 at 0.3 cM to SSR marker OSM89 to IB1 on chromosome 12 accounting for 5.44%; and qBLAST12.2 at 0.3 and 0.1 cM to SSR marker OSM89 to IB49 and ID1 on chromosome 12 accounting for 9.7% and 10.18% of phenotypic variation, respectively. This study demonstrates the usefulness of tagging blast QTLs using physiological races by multiple interval mapping. Additional keywords: blast, infection type, SSR markers

Last Modified: 9/10/2014
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