Author
Bassett, Carole | |
Wisniewski, Michael | |
MALNOY, M - Iasma Research Center | |
Norelli, John | |
VELASCO, RICCARDO - Iasma Research Center |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 7/30/2010 Publication Date: 8/12/2010 Citation: Bassett, C.L., Wisniewski, M.E., Malnoy, M., Norelli, J.L., Velasco, R. 2010. The CBF gene family in apple (malus x domestica Borkh.). Meeting Abstract. Interpretive Summary: Technical Abstract: Many vascular plants have evolved mechanisms for protecting themselves from freeze damage. One of the key pathways controlling higher plant responses to low temperature involves a family of genes which belong to the AP2 domain class of transcription factors. The promoters of many genes involved in abiotic stress responses contain specific elements known as cold repeat (C-repeat)/dehydration response elements (DRE) to which these transcription factors (called CF/DREB for C-repeat or dehydration responsive binding factors) bind to regulate transcription. In Arabiodpsis thaliana, there are four members of this family which regulate cole (CBF1, 2 and 3) and dehydration (CBF4) stress responses. We have idnetified seven genes from 'Golden Delicious' apple (Whole Genome Sequesnce project, FEM-IASMA, Italy) encoding AP2 genes homologous to the Arabidopsis CBF genes. Five of these genes appear to encode full length sequences corresponding primarily to the Arabidopsis CBF2 and 4 genes. Two of the apple genes encode polypeptides truncated at the N-terminus, due to alterations in their sequences which eliminate the 5'-most ATG. We present an analysis of this family and comparison to the CBF/DREB1 genes of Arabidopsis. |