IMPACT OF EARLY DIETARY FACTORS ON CHILD DEVELOPMENT AND HEALTH
Location: Arkansas Children Nutrition Center
Title: Enhanced mammary progesterone receptor-A isoform activity in the promotion of mammary tumor progression by dietary soy in rats
| Dave, Bhuvanesh - |
| Wynne, Rebecca - |
| Su, Ying - |
| Korourian, Soheila - |
| Chang, Jenny - |
| Simmen, Rosalia - |
Submitted to: Nutrition and Cancer
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 15, 2010
Publication Date: August 1, 2010
Citation: Dave, B., Wynne, R., Su, Y., Korourian, S., Chang, J.C., Simmen, R.C. 2010. Enhanced mammary progesterone receptor-A isoform activity in the promotion of mammary tumor progression by dietary soy in rats. Nutrition and Cancer. 62(2):774-782.
Interpretive Summary: Dietary intake of soy foods can be beneficial to women’s health, especially in the prevention of adult onset of breast cancer. However, there may be conditions under which soy consumption may have adverse effects. In this study, using a rat model, we show that breast cancer tumors that are exposed to dietary soy may lead to more advanced tumors. We show that this may be due to changes in the expression of progesterone receptors, which can alter the biology of the mammary tissue. Thus, diet may alter tumor outcome and must be strongly considered in women afflicted with breast cancer.
Dietary contribution to breast cancer risk, recurrence, and progression remains incompletely understood. Increased consumption of soy and soy isoflavones is associated with reduced mammary cancer susceptibility in women and in rodent models of carcinogenesis. In rats treated with N-Methyl-N-Nitrosourea, dietary intake of soy protein isolate (SPI) reduced mammary tumor occurrence, but increased incidence of more invasive tumors in tumored rats, relative to the control diet Casein. Here we evaluated whether mammary tumor progression in tumor-bearing rats lifetime exposed to SPI is associated with deregulated progesterone receptor (PR) isoform expression. In histological normal mammary glands of rats with invasive ductal carcinoma lesions, PR-A protein levels were higher for SPI- than Casein-fed rats while PR-B was undetectable for both groups. Increased mammary PR-A expression was associated with higher Transforming Growth Factor-beta 1, Stanniocalcin-1, and CD44 transcript levels; lower E-cadherin and estrogen receptor-alpha expression; and reduced apoptotic status in ductal epithelium. Serum progesterone (ng/ml) (CAS: 25.94±3.81; SPI: 13.19±2.32) and estradiol (pg/ml) (CAS: 27.9±4.49; SPI: 68.48±23.87) levels differed with diet. However, sera from rats of both diet groups displayed comparable mammosphere-forming efficiency in human MCF-7 cells. Thus, soy-rich diets may influence the development of more aggressive tumors by enhancing PR-A-dependent signaling in pre-malignant breast tissues.