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United States Department of Agriculture

Agricultural Research Service

Research Project: COTTON GENOMICS AND GENETIC ANALYSIS

Location: Crop Germplasm Research

Title: Portable DNA markers tailored for systematic characterization of Gossypium germplasm

Authors
item Yu, John
item Fang, David
item Ulloa, Mauricio
item Percy, Richard
item Kohel, Russell
item Hinze, Lori
item Frelichowski, James
item Cho, Jaemin
item Campbell, Benjamin
item Chee, Peng -
item Zhang, Jinfa -
item Abdurakhmonov, Ibrokhim -
item Abdukarimov, Abdusattor -
item Jones, Don -

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 10, 2009
Publication Date: January 9, 2010
Citation: Yu, J., Fang, D.D., Ulloa, M., Percy, R.G., Kohel, R.J., Hinze, L.L., Frelichowski, J.E., Cho, J., Campbell, B.T., Chee, P., Abdurakhmonov, I., Abdukarimov, A., Jones, D.C. 2010. Portable DNA markers tailored for systematic characterization of Gossypium germplasm [abstract]. In: Plant and Animal Genome Conference Proceedings, January 9-13, 2010, San Diego, California. P149.

Technical Abstract: Many small-scale ad-hoc studies on characterization of Gossypium germplasm have been conducted that use different sets of markers. Coordination with the cotton community is needed to reach a consensus on the appropriate initial set of DNA markers. In consultation with the cotton community, a set of 104 portable DNA markers were identified to provide a common basis for systematic characterization of cotton germplasm collections in the U.S. and throughout the world. The 104 PCR-based SSR markers of different origins were carefully developed and/or selected from the saturated molecular map of tetraploid cotton. These markers were evenly distributed on each of the 26 cotton chromosomes with every chromosome arm having 2 markers at approximately 30 cM intervals. Each of these markers was examined on a standardized germplasm panel consisting of 12 diverse Gossypium genotypes. Cotton germplasm accessions with unique DNA profiles may be investigated in detail with additional DNA markers for new genes. This set of DNA markers is presented to serve as an initial set of core DNA markers for global germplasm characterization. The initial core set can be modified and expanded as new DNA markers such as SNPs are developed, characterized, and located in the cotton genome.

Last Modified: 7/22/2014
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