Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Micropropagation and Cryopreservation: Alternative Techniques for Conserving Plant Genetic Resources

Author
item Reed, Barbara

Submitted to: Plant Growth Regulator Society of America Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2010
Publication Date: July 21, 2010
Citation: Reed, B.M. 2010. Micropropagation and Cryopreservation: Alternative Techniques for Conserving Plant Genetic Resources. Plant Growth Regulator Society of America Meeting. p. 58-64.

Interpretive Summary: Vegetatively propagated crops must be maintained as growing plants in fields or greenhouses. Growing plants are often at risk of loss from disease, environmental hazards or weather so it is important to have secure secondary collections in place. Micropropagation (tissue culture) and cryopreservation (storage in liquid nitrogen) are used for backup of the temperate fruit, nut and specialty crops held at the U.S. Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR) near Corvallis Oregon. Tissue culture collections of over 1200 accessions of 8 genera including many species and cultivars are held in medium-term storage as shoot cultures at 4°C or in long-term storage as meristems in liquid nitrogen. The diversity of the collections provides multiple challenges to micropropagation. For example the 192 accession in-vitro pear collection includes 19 species and 154 named cultivars. Research in progress includes optimization of growth media and storage conditions for the wide range of genetic diversity in the collection as well as improved cryopreservation protocols. Cryopreservation techniques were developed for the genera at NCGR and many accessions are in long-term storage in liquid nitrogen.

Technical Abstract: The genetic resources of vegetatively propagated crops must be maintained as growing. Growing plants in field or greenhouse collections may be at risk of loss from disease, and environmental hazards or weather so it is important to have secure secondary collections in place. Micropropagation and cryopreservation are used for backup of the temperate fruit, nut and specialty crops held at the U.S. Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR) near Corvallis Oregon. Tissue culture collections of over 1200 accessions from 8 genera including many species and cultivars are held as shoot cultures in low temperature storage (4°C) or as meristems held in liquid nitrogen. The genetic diversity of the collections poses multiple challenges. For example, the 192 pear accessions maintained in vitro include 19 species and 154 named cultivars. Research on optimizing growth media and storage conditions for the wide range of genetic diversity in the collection as well as improved cryopreservation protocols is in progress. Cryopreservation techniques were developed for many of the genera at NCGR and many accessions are in long-term storage in liquid nitrogen.

Last Modified: 7/28/2014
Footer Content Back to Top of Page