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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVEMENT OF PEST RESISTANCE AND QUALITY TRAITS OF SOYBEAN

Location: Corn, Soybean and Wheat Quality Research Unit

Title: Identification of Proteins Differentially Regulated in Response to Soybean Aphid Infestation of Soybean Near Isogenic Lines differing in Aphid Resistance

Authors
item Brechenmacher, Laurent -
item Nguyen, Tran Hong Nha -
item Wang, Jun -
item Jun, Tae Hwan -
item Mian, Rouf
item Stacey, Gary -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 30, 2010
Publication Date: N/A

Technical Abstract: The soybean aphid, a plant sap sucking insect, has become an important soybean pest in the USA and infestation of soybean by this insect can lead to significant yield losses. The Rag2 gene of soybean, providing resistance to soybean aphid biotypes I (IL) and II (OH), was identified by researchers in Ohio. A proteomic analysis was performed on BC4 near isogenic lines (NILs) with the Rag2 gene for resistance or rag2 for susceptibility to the soybean aphid to unravel mechanisms of aphid resistance. Soybeans were grown in an environment controlled greenhouse to the V1 (first trifolitate) stage and infested with 20 adult soybean aphids of biotype II. Leaves were collected in four replicates at 0, 2, 4, 8, 24, 36, 48, 72 and 96 hours after infestation. Proteins from 4 biological replicates of Rag2 and rag2 NILs 48h after aphid infestation were extracted, separated by 1D gel electrophoresis and stained by colloidal Coomassie blue. Each line of the gel was divided into 12 bands and digested by Trypsin. Tryptic peptides were analyzed on an Agilent 6520 Quadruplole-Time of Flight-Mass spectrometer using a 43 mm chip. Fourteen hundred and eighty proteins were detected in at least 3 biological replicates of Rag2 or rag2 NILs. Statistical analysis identified 112 proteins differentially regulated between NILs 48h after aphid infestation. Efforts are continuing to analyze samples from other time points. The ultimate goal is to identify proteins involved in soybean aphid resistance conferred by the Rag2 gene.

Last Modified: 7/28/2014
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