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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #251976
Title: Current status and perspectives of unificiation of Glu-3 nomenclature systems in common wheat

Author
item IKEDA, T. - National Agricultural Research Organization - Japan (NARO)
item BRANLARD, G. - Institut National De La Recherche Agronomique (INRA)
item PENA, R. - International Maize & Wheat Improvement Center (CIMMYT)
item TAKATA, K. - National Agricultural Research Organization - Japan (NARO)
item LIU, L. - International Maize & Wheat Improvement Center (CIMMYT)
item HE, Z. - International Maize & Wheat Improvement Center (CIMMYT)
item LUKOW, O. - Agriculture And Agri-Food Canada
item APPELBEE, M. - Longreach Plant Breeders
item Hurkman Ii, William
item LERNER, S. - National University Of Central Buenos Aires Province
item KOLMAN, M. - National University Of Central Buenos Aires Province
item ROGERS, W. - National University Of Central Buenos Aires Province

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2010
Publication Date: 6/1/2010
Citation: Ikeda, T.M., Branlard, G., Pena, R.J., Takata, K., Liu, L., He, Z., Lukow, O., Appelbee, M., Hurkman Ii, W.J., Lerner, S.E., Kolman, M.A., Rogers, W.J. 2010. Current status and perspectives of unificiation of Glu-3 nomenclature systems in common wheat. 8th International Wheat Conference (IWC), St. Petersburg, Russia, June 1-4, 2010.

Interpretive Summary:

Technical Abstract: Low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat is one of the critical determinants of gluten properties. However, the nomenclature of Glu-3 encoding LMW-GSs has not been consistent among laboratories, due to the complexity of the LMW-GSs and the distinct separation methods used by different researchers. It is very important to unify the nomenclature systems in current use, to facilitate the sharing of information about the effects of individual LMW-GS on gluten properties. We therefore shared cultivars having various Glu-A3, Glu-B3 and Glu-D3 alleles from various countries. Using 1D SDS-PAGE, 2D and MS analyses, we found differences in nomenclature particularly for Glu-A3 and Glu-B3, including new Glu-3 alleles. For 1D SDS-PAGE, various running condition were compared to identify these alleles efficiently. We propose a new list of standard cultivars representing Glu-3 alleles and standard SDS-PAGE methods to identify them.