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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND BIOLOGICAL DETERMINANTS OF AVIAN TUMOR VIRUS SUSCEPTIBILITY

Location: Avian Disease and Oncology Laboratory

Title: Diagnosis of Marek's Disease From a Japanese Quail (Coturnix Japonica) Using Paraffin-embedded Liver

Authors
item Dunn, John
item Southard, Teresa -
item Cooper, Coreen -
item Kiupel, Matti -
item Witter, Richard

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2010
Publication Date: August 1, 2010
Citation: Dunn, J.R., Southard, T., Cooper, C., Kiupel, M., Witter, R.L. 2010. Diagnosis of Marek's Disease From a Japanese Quail (Coturnix Japonica) Using Paraffin-embedded Liver [abstract]. In: American Association of Avian Pathologists Symposium and Scientific Program, August 1-4, 2010, Atlanta, Georgia. Paper No. 9389.

Technical Abstract: A single paraffin-embedded liver section was submitted from a research flock of Japanese quail that had revealed focal infiltrations of immature lymphocytes within multiple visceral organs. Tumor cells were characterized as T-cells positive for Marek's disease virus (MDV) pp38 antigen by IHC dual staining. Confirmation of MDV infection by PCR from DNA isolated from the paraffin-block provided supportive evidence, although only successfully with PCR products <1.41 kb in length, likely due to DNA damage caused by fixation. This case report provides an example of validation for tumor virus advanced diagnostic techniques using a non-ideal sample type.

Last Modified: 10/1/2014
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