ARS | Publication request: Update on the Search for New Toxicants and Methods for Control

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 9, 2010
Publication Date: N/A

Technical Abstract: The search for new active ingredients for vector control involves two main approaches. The first is the screening of large numbers of experimental compounds (synthetic and natural products) using a primary high throughput screen (HTS) bioassay using Aedes aegypti larvae. Highly active compounds are then evaluated in secondary topical adult (Ae. aegypti) bioassays. Bioassay data are analyzed using structural-activity relationship (SAR) methods to identify desirable characteristics. Analogs of active compounds are then evaluated for adulticidal activity. The second approach is the targeting of critical genes/proteins to develop molecular (genetic) biopesticides through RNA interference (RNAi). Constructs are designed, produced and evaluated for efficacy. Preliminary results suggest that: (1) chemical screening using bioassays combined with SAR analysis is useful in identifying potential toxicants for vector control and (2) targeting critical genes/proteins through RNAi offers exciting possibilities for novel pesticide development.