Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED PLANT GENETIC RESOURCES FOR PASTURES AND RANGELANDS IN THE TEMPERATE SEMIARID REGIONS OF THE WESTERN U.S.

Location: Forage and Range Research

Title: Phenotypic and genetic characterization of western prairie clover collections from the western USA

Authors
item Bhattarai, Kishor -
item Bushman, Shaun
item Johnson, Douglas
item Carman, John -

Submitted to: Rangeland Ecology and Management
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 11, 2010
Publication Date: November 1, 2010
Citation: Bhattarai, K., Bushman, B.S., Johnson, D.A., Carman, J.G. 2010. Phenotypic and genetic characterization of western prairie clover collections from the western USA. Rangeland Ecology and Management. 63:696-706.

Interpretive Summary: Few North American legumes are available for rangeland revegetation in the semi-arid western USA. Western prairie clover (Dalea ornata [Douglas] Eaton & Wright) is a perennial legume with desirable characteristics and is distributed in the Great Basin, Snake River Basin, and southern Columbia Plateau. Understanding the genetic and ecotypic variability of this species is a prerequisite for developing populations suitable for revegetation purposes. To address this need, we established two field plots of western prairie clover that originated from 24 sites in Idaho, Oregon, and Washington. Significant variation was detected among the collections for all traits measured. Among the measured traits, flowering time was correlated with collection-site temperature and elevation, which suggests adaptive significance. Genetic population structure estimates from 474 amplified-fragment length polymorphism markers resulted in two distinct, genetically differentiated groups and a third admixed group. These groups also differed in flowering date, inflorescence weight, and plant height. Significant positive correlations were observed between phenotypic and genetic distance matrices (r = 0.35, P = 0.003), phenotypic and geographic distance matrices (r = 0.35, P = 0.002), and genetic and geographic distance matrices (r = 0.32, P = 0.003). Based on these results, we recommend that two germplasm sources of western prairie clover be developed for use in rangeland revegetation, one from the Deschutes River watershed and the other encompassing the remaining range of collection sites.

Technical Abstract: Few North American legumes are available for rangeland revegetation in the semi-arid western USA. Western prairie clover (Dalea ornata [Douglas] Eaton & Wright) is a perennial legume with desirable characteristics and is distributed in the Great Basin, Snake River Basin, and southern Columbia Plateau. Understanding the genetic and ecotypic variability of this species is a prerequisite for developing populations suitable for revegetation purposes. To address this need, we established two field plots of western prairie clover that originated from 24 sites in Idaho, Oregon, and Washington. Significant variation was detected among the collections for all traits measured. Among the measured traits, flowering time was correlated with collection-site temperature and elevation, which suggests adaptive significance. Genetic population structure estimates from 474 amplified-fragment length polymorphism markers resulted in two distinct, genetically differentiated groups and a third admixed group. These groups also differed in flowering date, inflorescence weight, and plant height. Significant positive correlations were observed between phenotypic and genetic distance matrices (r = 0.35, P = 0.003), phenotypic, and geographic distance matrices (r = 0.35, P = 0.002), and genetic and geographic distance matrices (r = 0.32, P = 0.003). Based on these results, we recommend that two germplasm sources of western prairie clover be developed for use in rangeland revegetation, one from the Deschutes River watershed and the other encompassing the remaining range of collection sites.

Last Modified: 7/30/2014
Footer Content Back to Top of Page