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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR ANALYSIS OF VIRULENCE DETERMINANTS OF SELECT BACTERIA IN FISH DISEASES

Location: Aquatic Animal Health Research

Title: cDNA sequences of channel catfish (Ictalurus punctatus Rafinesque, 1818) annexin A2, A4, A5 and A11

Authors
item Yeh, Hung-Yueh
item Klesius, Phillip

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: December 16, 2009
Publication Date: December 16, 2009
Citation: Yeh, H., Klesius, P.H. 2009. cDNA sequences of channel catfish (Ictalurus punctatus Rafinesque, 1818) annexin A2, A4, A5 and A11. Genbank. Accession numbers A2 - FJ803363, A4 - FJ803364, A5 - FJ803365 and A11 - FJ803366.

Technical Abstract: Annexins, a protein superfamily, are ubiquitous, and play many important roles in immuno-physiological processes. In this report, we cloned and sequenced channel catfish orthologs to human annexin A2, A4, A5 and A11. Total RNA from tissues was extracted and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the annexin transcripts. Each annexin nucleic acid sequence had 5’-untranslated region (UTR), an open reading frame (ORF) and 3’-UTR. Each ORF appears to encode 337, 321, 317 and 482 amino acid residues for A2, A4, A5 and A11, respectively. By comparison with the human counterparts, each channel catfish annexin also had four conserved 70 amino-acid-residue repeats. These results provide important information for further elucidating annexin functions in channel catfish.

Last Modified: 7/25/2014
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