Location: Horticultural Crops Research
Title: Virus Survey in Strawberry in the United States and Canada Author
Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: December 18, 2009
Publication Date: December 18, 2009
Citation: Martin, R.R. 2009. Virus survey in strawberry in the United States and Canada. Great Lakes Fruit, Vegetable, and Farm Market Expo Program Book. Technical Abstract: In an effort to determine the distribution of strawberry viruses in the United States and Canada, approximately 1500 samples were collected and either brought back or shipped to the USDA-ARS laboratory in Corvallis between 2002 and 2007. RNA was extracted from leaf tissue and archived at -80C for subsequent uses. During the same time, RT-PCR tests were developed for most known strawberry viruses. For this study 275 samples representing the major strawberry production areas in the US and Canada were tested for: Apple mosaic virus (ApMV), Beet pseudo yellows (BPYV), Fragaria chiloensis latent (FClLV), Strawberry chlorotic fleck associated virus (SCFaV), Strawberry crinkle (SCV), Strawberry latent ringspot (SLRSV), Strawberry mottle (SMoV), Strawberry mild yellow edge (SMYEV), Strawberry necrotic shock (SNSV), Strawberry pallidosis (SPaV) and Strawberry vein banding (SVBV) and Tobacco streak (TSV) viruses, as well as a housekeeping gene used as an internal control by RT-PCR. The Pacific Northwest had the highest rates of infection with the aphid-borne viruses but was virtually free of the whitefly transmitted viruses. In contrast, California, the southeastern US, the northeastern US and the Midwest had aphid and whitefly transmitted viruses in about equal numbers, with the Midwest having the lowest overall incidence of virus infection. BPYV was only found in the samples from CA and the southeastern US in the samples used in these tests, but has been detected in samples from Maryland in previous studies. In the Pacific Northwest, fields with aphid control had very low incidence of virus infection compared to nearby fields without aphid control. Also virus incidence was much lower in Oregon than in northern Washington or British Columbia, which matches the relative populations of the strawberry aphid.