Submitted to: United States-Japan Cooperative Program in Natural Resources
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 2009
Publication Date: December 4, 2009
Citation: Nicholson, T.L., Kukielka, D., Wang, D., Faaberg, K.S. 2009. Detection Limits of PRRSV and SIV Using Pan Viral Microarray [abstract]. 45th Meeting of the United States-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health, December 2-4, 2009, Tokyo, Japan. p. 21. Technical Abstract: Several factors have recently converged elevating the need for highly parallel diagnostic platforms that have the ability to detect many known, novel, and emerging pathogenic agents simultaneously. Panviral DNA microarrays represent the most robust approach for massively parallel viral surveillance and detection. The Virochip is a panviral DNA microarray that is capable of detecting all known viruses, as well as novel viruses related to known viral families in a single assay and has been extensively used to successfully identify known and novel viral agents in clinical human specimens. However, the usefulness and the sensitivity of the Virochip platform has not been tested on a set of clinical veterinary specimens with the high degree of genetic variance that is frequently observed in swine virus field isolates. In this report we investigated the utility and sensitivity of the Virochip to positively detect swine viruses in both cell culture derived samples and clinical swine samples. The swine viruses chosen in this investigation represent the most important viral pathogens in terms of impact on both health and economic loss to the US swine producers.