NEWCASTLE DISEASE EPIDEMIOLOGY, PATHOGENESIS, AND CONTROL
Location: Exotic and Emerging Avian Viral Diseases Research Unit
Title: Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: December 30, 2009
Publication Date: August 1, 2010
Citation: Susta, L., Miller, P.J., Hu, S., Liu, X., Rue, C.A., Afonso, C.L., Brown, C.C. 2010. Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain [abstract}. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9392.
Four-week-old white Leghorn chickens were inoculated intraconjunctivally with either a virulent recombinant clone of Newcastle disease virus (NDV), rZJ1, or a modified rZJ1, containing a green fluorescent protein (GFP), (rZJ1-GFP). The ZJ1 parent strain was responsible for NDV outbreaks in Southern China and belongs to genotype VIId. Subsequent to inoculation, birds were monitored and euthanized sequentially with collection of tissues for histopathological examination, immunohistochemistry (IHC) for viral nucleoprotein or GFP and in situ hybridization to detect viral replication. Disease with rZJ1 infection was severe with acute systemic disease, high mortality rates, necrosis of lymphoid tissues, and detection of viral nucleoprotein in multiple tissues. With rZJ1-GFP, viral distribution was similar, but disease was less severe and production of GFP was confirmed by positive IHC labeling.