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United States Department of Agriculture

Agricultural Research Service

Research Project: PREVENTION AND TREATMENT OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS (MAP) INFECTION IN MICE BY LACTOBACILLUS ACIDOPHILUS NP51 Title: Prevention of Mycobacterium avium subsp. paratuberculosis (MAP) Infection in BALB/c Mice by Feeding Probiotic Lactobacillus acidophilus NP-51

Authors
item Osman, A -
item Stabel, Judith
item Hostetter, M -
item Nettleton, D -
item Beitz, D -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 10, 2010
Publication Date: April 24, 2010
Citation: Osman, A.M., Stabel, J.R., Hostetter, M., Nettleton, D., Beitz, D.C. 2010. Prevention of Mycobacterium avium subsp. paratuberculosis (MAP) Infection in BALB/c Mice by Feeding Probiotic Lactobacillus acidophilus NP-51 [abstract]. Experimental Biology. p. 102.5.

Technical Abstract: The objective of this study was to examine effects of feeding Lactobacillus acidophilus strain NP51 to mice challenged with Mycobacterium avium subspecies paratuberculosis (MAP). Mice were randomized to ten treatment groups; sentinels, control, heat-killed MAP, viable MAP, heat-killed NP51, viable NP51, heat-killed NP51 plus heat-killed MAP, heat-killed NP51 plus viable-MAP, viable-NP51 plus heat-killed MAP, viable-NP51 plus viable-MAP. Mice were fed 1 × 10**6 CFU of NP51· mice-1 · day-1. On day 45, mice were challenged with 1 × 10**8 CFU of MAP intraperitonealy. We hypothesized that feeding NP51 would increase Th-1 responses and decrease progression of Johne’s disease in mice. Ten mice from each group were euthanized on days 45, 90, 135 and 180. Supernatante from in vitro splenocyte culture was examined for IFN-gamma production. Feeding Heat-killed NP51 to heat-killed MAP- or viable MAP-infected mice increased interferon (IFN)-gamma by 1.4- and 4-fold (44.32 and 127.14 vs. 31.69 pg/mL, respectively) over that of heat-killed MAP-infected control. Similarly, feeding viable-NP51 to heat-killed MAP- or viable MAP-infected mice increased IFN-gamma by 3- and 4- fold (98.6 and 130.68 vs. 31.69, respectively) over that of heat-killed MAP-infected control. These data suggest that feeding NP51 can simulate IFN-gamma production and may prevent progression of Johne’s disease in mice.

Last Modified: 10/20/2014
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