ARS | Publication request: Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 18, 2009
Publication Date: March 1, 2010
Citation: Batt Throne, S.B., Wan, D., Doi, R.H., Wong, D. 2010. Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae. Meeting Abstract, 2010; CELL Poster Session; Poster #57 (Paper ID 18330).

Technical Abstract: Enzyme systems that digest the cellulose and hemicellulose in plant cell walls have potential value in the biorefining of feedstocks such as crop residues, straws, and grasses to fuel ethanol and other bioproducts. Clostridium cellulovorans is a useful source of biomass-degrading enzymes because this anaerobic bacterium produces cellulosomes – extracellular complexes of enzymes that degrade cellulose. The genes of several cellulosomal and non-cellulosomal enzymes were cloned into yeast expression vectors and transformed into the model eukaryotic organism Saccharomyces cerevisiae. Active endoglucanase, exoglucanase, and xylanase enzymes were constitutively expressed and secreted. These enzymes are being studied as prospective components of enzyme complexes for high-efficiency digestion of cellulosic and hemicellulosic biomass to fermentable sugars for biofuel production.