|Bae, Jae-Han -|
|Suh, Min-Jung -|
|Kim, Beom-Soo -|
|Lee, In-Jung -|
|Kim, In-Hwan -|
|Kim, Hak-Ryul -|
Submitted to: New Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 15, 2009
Publication Date: February 19, 2010
Citation: Bae, J., Suh, M., Kim, B., Hou, C.T., Lee, I., Kim, I., Kim, H. 2010. Optimal production of 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3. New Biotechnology. 27:352-357. Interpretive Summary: Previously, we discovered a new microbial culture Pseudomonas aeruginosa PR3 that produced 7,10-dihydroxy-fatty acid from oleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, coating materials, and possible physiologically active agents. Now we found that strain PR3 also coverts palmitoleic acid to 7,10-dihydroxy-8(E)-hexadecenoic acid. These findings raise the possibility of using microbial bioconverted fatty acid products as specialty chemicals and bioactive agents to control pathogenic bacteria and several plant fungal diseases.
Technical Abstract: The hydroxylation of unsaturated fatty acids by bacterial strains is one type of value-adding bioconversion process. This process generates new hydroxy fatty acids (HFA) carrying special properties such as higher viscosity and reactivity compared with normal fatty acids. Among microbial strains tested for HFA production, Pseudomonas aeruginosa PR3 is well known to convert unsaturated fatty acids into several hydroxyl fatty acids. Previously we reported that strain PR3 could produce a novel hydroxy fatty acid 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid. In this study, we focused on optimization of environmental conditions for DHD production. Optimal carbon and nitrogen sources for DHD production were fructose and yeast extract, respectively. Optimal initial medium pH and incubation temperature were pH 8.0 and 30**oC and magnesium ion was required for DHD production. Substrate concentration and time of substrate addition were also optimized. Under optimized conditions, maximal DHD production was 1600 mg per liter representing 26.7% conversion yield.