Technical Abstract: A new Parasitorhabditis species with males and females was discovered from the southern pine beetle Dendroctonus frontalis and its galleries in loblolly pine growing in Mississippi. Females of the new species have a cupola-shaped tail with a small spike; males possess a 2 + (3+2) + 3 ray pattern on the tail fan and distinctive gubernaculum. Parasitorhabditis frontali n. sp. has some similarities to Parasitorhabditis hylurgi from Hylurgops pinifex in New York, USA, P. terebranus from Dendroctonus terebrans in Texas, USA, P. ateri isolated in Germany from the beetle Hylastes ater, and P. malii from Scolytus mali within the republic of Georgia. Many of the approximately 44 species descriptions of Parasitorhabditis are relatively inaccessible due to language or journal obscurity. Parasitorhabditis frontali n. sp. was initially grown on Malt Extract (ME) agar with its own microbial contaminants that included a bacterium, yeast and another fungus. The nematode also grew and reproduced after chunks of ME agar with nematodes and microbial contaminants were transferred to water agar. It was killed by E. coli on NGM agar plates commonly used to raise other Rhabditida. Drawings of diagnostic anatomy and low-temperature SEM images of bodies, heads, and tails are provided for cultured specimens.