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United States Department of Agriculture

Agricultural Research Service

Research Project: Alternatives to methyl bromide soil fumigation for vegetable and floriculture production

Location: Subtropical Plant Pathology Research

Title: Biological Evaluation and comparison of four Florida isolates of Meloidogyne floridensis

Authors
item Stanley, Jason -
item Brito, Janete -
item BURELLE, NANCY
item Frank, J. H. -
item Dickson, Donald -

Submitted to: Nematropica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 20, 2010
Publication Date: December 1, 2009
Citation: Stanley, J.D., Burelle, N.K., Brito, J.A., Frank, J., Dickson, D.W. 2009. Biological Evaluation and comparison of four Florida isolates of Meloidogyne floridensis. Nematropica. 39:255-271.

Technical Abstract: A four-year study was conducted in Florida to characterize the morphology, enzymatic profile, and host preference of four isolates of the peach root-knot nematode, Meloidogyne floridensis, a recently described species of root-knot nematode that infects and reproduces on the peach rootstock cultivars Guardian, Nemaguard, Nemared, and Okinawa. These rootstocks are resistant to M. incognita and M. javanica. No morphological or biochemical differences were observed among the four isolates. Each isolate showed some mean variability in morphometrics values, but overlapped in their range values (P > 0.05). In Total, 1,027 females extracted from peach, pepper, tobacco, and tomato did not differ in their isozyme phenotype for esterase and malate dehydrogenase, and matched those reported in the original description. In host differential tests all four isolates of M. floridensis exhibited the same reaction as that of M. incognita race 2, with hosts being pepper, tobacco, tomato and watermelon, and nonhosts being cotton and peanut. In comparative host status studies, both root-knot nematode resistant and susceptible peach cultivars were good hosts for all four isolates. In comparative host status studies of both resistant and susceptible cultivars of corn, pepper, soybean, and tomato, all four isolates of M. floridensis reproduced poorly but were able to overcome the resistance of the Mi-1 gene in Crista tomato. Two isolates reproduced poorly but were able to overcome the N gene resistance in the pepper cv. Charleston Belle, whereas two isolates reproduced well on this cultivar. Both the root-knot nematode resistant corn cv. Mp 710 and susceptible cv. Dixie 18 were good hosts for all four isolates whereas; both the resistant soybean cv. Forrest and susceptible cv. S64-J1 were nonhosts for all four isolates of M. floridensis.

Last Modified: 7/25/2014
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