Title: Development of long-term preservation and re-activation techniques for ANAMMOX bacteria Authors
Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 12, 2009
Publication Date: October 12, 2009
Citation: Rothrock Jr, M.J., Vanotti, M.B., Szogi, A.A. 2009. Development of long-term preservation and re-activation techniques for ANAMMOX bacteria [abstract]. American Society of Agronomy-Crop Science Society of America-Soil Science Society of America International Annual Meeting Abstracts. Technical Abstract: ANAMMOX are slow growing biofilm-forming bacteria responsible for the anaerobic oxidation of ammonia to produce nitrogen gas and nitrate. ANAMMOX-based bioreactors are being used to effectively treat high ammonia concentrations in industrial, municipal and agricultural wastewaters. Usually, fresh sludge is needed to start a new bioreactor because long-term storage and reactivation of ANAMMOX sludge is difficult. Currently, there is a paucity of methods for long-term preserving and quickly re-activating ANAMMOX bacteria. The objective of our research was to compare a variety of preservation methods on freshly collected ANAMMOX and test sludge reactivation after storage. Several different techniques will be presented for preservation and potential long-term storage of ANAMMOX sludge samples for later use as “seed” inocula for new bioreactors.