Title: Harnessing the sorghum genome sequence:development of a genome-wide microsattelite (SSR) resource for swift genetic mapping and map based cloning in sorghum Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 25, 2009
Publication Date: February 28, 2009
Citation: Burow, G.B., Franks, C.D., Xin, Z., Burke, J.J. 2009. Harnessing the sorghum genome sequence:development of a genome-wide microsattelite (SSR) resource for swift genetic mapping and map based cloning in sorghum[abstract]. 2009 Sorghum Improvement Conference of North America. February 25-28, 2009, Grapevine, Texas. CDROM. Technical Abstract: Sorghum is the second cereal crop to have a full genome completely sequenced (Nature (2009), 457:551). This achievement is widely recognized as a scientific milestone for grass genetics and genomics in general. However, the true worth of genetic information lies in translating the sequence information into developing methods and technologies for sorghum improvement thru various breeding strategies (Sasaki and Antonio, (2009): Nature (457:547-548). To harness the wealth of information afforded by whole genome sequence of sorghum, a new set of highly informative and easy to use simple sequence repeat (SSRs) DNA markers have been developed. A total of 620 SSRs were mined from the sorghum genome sequence (www.phytozome.net), at an interval of ~1million nucleotides, and were designed to cover the whole genome. The information on these SSR markers will be made available through a web accessible platform. A subset of the SSR markers had been tested for swift mapping of the blmc or bloomless mutation and that of twin seeded trait. Further characterization and applications of these new markers will be discussed. It is anticipated that with application of easy to use SSR markers, faster advances in sorghum breeding will be realized.