NUTRITIONAL REGULATION OF CELL AND ORGAN GROWTH, DIFFERENTIATION, AND DEVELOPMENT
Location: Children Nutrition Research Center (Houston, Tx)
Title: Acute effects of enteral leucine supplementation of a low protein diet on muscle protein synthesis in neonatal pigs
| Torrazza, Robert - |
| Suryawan, Agus - |
| Orellana, Renan - |
| Gazzaneo, Carolina - |
| Nguyen, Hanh - |
| Almonaci, Rose - |
| Davis, Teresa - |
Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: February 20, 2009
Publication Date: April 2, 2009
Citation: Torrazza, R.M., Suryawan, A., Orellana, R., Gazzaneo, C., Nguyen, H., Almonaci, R., Davis, T. 2009. Acute effects of enteral leucine supplementation of a low protein diet on muscle protein synthesis in neonatal pigs [abstract]. Federation of American Societies for Experimental Biology Conference: Today's Research: Tomorrow's Health. Session: Piglet model of perinatal growth, nutrition and digestive physiology, April 18-22, 2009, New Orleans, Louisiana. Electronic Abstract: 23(1) Abstract No. 33.1.
Protein synthesis and eukaryotic initiation factor (eIF) activation are increased in skeletal muscle of neonatal pigs parenterally infused with insulin and amino acids (AA), particularly leucine. We hypothesized that enteral Leu supplementation of a low protein diets in neonatal pigs will acutely increase muscle protein synthesis to a rate similar to that achieved by feeding a high protein (HP) diet. Fasted 5-d-old piglets (n=12) were gavage fed at 0 and 60 min either: 1) low protein diet (LP), 2) low protein diet supplemented with Leu (LP+L) to equal HP diet, or 3) HP diet. Diets were isocaloric and lactose was equal. Fractional protein synthesis rates and translational initiation control mechanisms were examined 90 min after feeding. Muscle protein synthesis increased in the LP+L group compared to the LP group alone and did not differ from the HP group. LP+L and HP increased the phosphorylation of S6K1, eIF4G, and 4E binding protein-1 (4E-BP1), decreased inactive 4E-BP1>eIF4E complex abundance, and increased active eIF4E>eIF4G complex formation in muscle. There were no difference between groups in PKB phosphorylation. Our results suggest that low protein diets supplemented with leucine stimulate muscle protein synthesis to a rate similar to that achieved by high protein diets and this stimulation involves activation of mTOR downstream effectors.