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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR CHARACTERIZATION AND GASTROINTESTINAL TRACT ECOLOGY OF COMMENSAL HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN THE CHICKEN

Location: Poultry Microbiological Safety Research

Title: Phenotype microarray comparison of Campylobacter jejuni 11168 and Campylobacter coli 49941

Authors
item Line, John
item Hiett, Kelli

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: August 20, 2009
Publication Date: September 1, 2009
Citation: Line, J.E., Hiett, K.L. 2009. Phenotype microarray comparison of Campylobacter jejuni 11168 and Campylobacter coli 49941. Campylobacter Helicobacter and Related Organisms International Workshop. Sept. 1-5, 2009. Niigata, Japan.

Technical Abstract: Introduction: Campylobacter jejuni and coli are well established human food-borne pathogens. There is evidence that some commonly utilized selective media may bias recovery of the organisms based upon the nutrient and antibiotic content of the media. Phenotype microarray (PM) analysis enables profiles of the bacteria to be established utilizing approximately 2000 tests of nutrient requirements, as well as chemical and antibiotic resistances. Methods: Campylobacter strains were cultured from frozen stocks for 48h at 37oC on Brucella agar and transferred once prior to testing. Care was taken to carefully maintain microaerobic conditions at all times and inoculated PM plates were sealed in plastic barrier bags to prevent exposure to ambient atmospheric conditions during the assay. Incubation and data collection were conducted over a 72h period. Results: Carbon and nitrogen utilization by the genetically sequenced strains, C. jejuni 11168 and C. coli 49941, were initially compared. C. jejuni 11168 uniquely demonstrated enhanced utilization of citric and malic acids and rapid utilization of L-serine, while C. coli 49941 showed enhanced utilization of succinamic and propionic acids. Both strains demonstrated utilization of a number of compounds in common including ammonia, L-cysteine and, succinic, alpha-ketoglutaric and pyruvic acids among others. Conclusions: These data and further PM comparisons of chemical and antibiotic resistances should yield information enabling the creation of less biased selective media for Campylobacter spp. isolation.

Last Modified: 7/30/2014
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