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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #242291

Title: Tandem Repeat Stability in Escherichia coli O157:H7 is Dependent on Environmental Stress

Author
item Cooley, Michael
item Carychao, Diana
item Nguyen, Kimberly
item Whitehand, Linda
item Mandrell, Robert

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/10/2010
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Multilocus variable number tandem repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication, but changed after irradiation, elevated temperatures and starvation conditions. Plasmid, pO157, was lost frequently under these stresses. Environmental stresses may increase phylogenetic diversity measured by MLVA.