ARS | Publication request: A Genetic Linkage Map of Mycosphaerella Fijiensis, using SSR and DArT Markers

Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: January 17, 2009
Publication Date: March 17, 2009
Citation: Ferreira, C.F., Van Der Lee, T.J., Zapater, M.F., Carlier, J., Goodwin, S.B., Souza, M.T., Kema, G.J. 2009. A Genetic Linkage Map of Mycosphaerella Fijiensis, using SSR and DArT Markers. Fungal Genetics Conference Proceedings. Abstract No. 513.

Technical Abstract: Mycosphaerella fijiensis is the causal agent of black leaf streak or Black Sigatoka disease in bananas. This pathogen threatens global banana production as the main export Cavendish cultivars are highly susceptible. Previously a genetic linkage map was generated predominantly using anonymous AFLP markers. To assist genome assembly and the selection of markers for population analysis we generated a genetic linkage map of M. fijiensis using 87 SSR (Simple-Sequence Repeat) markers, 3 VNTR (Variable Number of Tandem Repeat) markers, the mating type (Mat) locus and 235 DArT (Diversity Arrays Technology) markers. The segregation of these markers was studied in 136 individuals derived form a cross between CIRAD086 (Cameroon, (Mat1-1) x CIRAD 139A (Colombia, Mat1-2). The genetic linkage map comprises 19 linkage groups covering 1417 cM. The arrays containing individual fragments of the genomic representation of M. fijiensis generated DArT markers with a 90% genotype call rate and 98.8% reliability score. In total, 87% of the markers could be positioned reliably with high LOD scores (LOD >10). Due to the excellent genome coverage and high quality we decided to sequence the DArT markers to align this genetic map with the genome sequence of CIRAD086, which will considerably contribute to the current genome assembly (http://genome.jgi- psf.org/Mycfi1/Mycfi1.home.html) of this important fungus.