|Kapur, Vivek -|
|Eda, Shigetoshi -|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: September 1, 2009
Publication Date: January 1, 2010
Citation: Bannantine, J.P., Paustian, M., Kapur, V., Eda, S. 2010. Proteome and Antigens of Mycobacterium avium subspecies Paratuberculosis. In: Behr, M.A., Collins, D.M., editors. Paratuberculosis Organism, Disease, Control. Wallingford, Oxfordshire, UK: CABI. p. 94-108. Technical Abstract: This chapter will review what is known about the proteome of this significant veterinary pathogen by discussing the use of various whole cell preparations and fractionated components thereof. With improvements in technology, many new modifications to the traditional ELISA assay have been introduced including flow cytometry and SERS-based assays. Also, new antigen extracts have been created that appear to improve on the current ELISA test, which has historically used a whole cell sonicated extract as the antigen. Particularly promising is the EV-ELISA extract that suggests sensitivities above 90%. Recombinant protein expression strategies have gained new momentum as well. The construction and use of the first protein array for M. avium subsp paratuberculosis (MAP) is one such application that has flowed from this strategy and it will be discussed at the end of this chapter.