Location: Mosquito and Fly Research Unit
Title: Multiple genes expressed in response to Magnesium stress of Culex quinquefasciatus larvae Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 5, 2009
Publication Date: N/A
Technical Abstract: It has been reported that magnesium is crucial to transmission of the virus in field populations of Culex nigripalpus and C. quinquefasciatus and also in the laboratory (Becnel et al. 2001). To understand why magnesium is essential mediated virus transmission and what kind of genes response to the early magnesium stimulation, we use suppression subtractive hybridization (SSH) to identify target transcripts to magnesium treatment in C. quinquefasciatus larvea. Subtraction was performed in both directions enriching for cDNAs differentially expressed between a non magnesium control and magnesium treatment. Magnesium treatment of C. quinquefasciatus larvea was carried out for 1 hr at 27 ºC. Clones from differentially expressed genes were evaluated by sequencing. Target transcripts up/down-regulated by magnesium included Culex pipiens quinquefasciatus troponin C, isocitrate dehydrogenase, allergen, cytochrome b5, chymotrypsinogen, apolipophorins, tryptase gamma, tropomyosin invertebrate, carboxylesterase, prolylcarboxypeptidase, as well as many other hypothetical protein genes. Magnesium can drastically alter the gene expression of a vector mosquito population, and understanding this process at the gene level can be used to develop novel control approaches of the transmission of baculoviruses in mosquitoes.