Title: Bean Pod Mottle Virus Spread in Insect Feeding Resistant Soybeans Authors
|Molineros, Julio - THE OHIO STATE UNIVERSITY|
|Vacha, Jean - THE OHIO STATE UNIVERSITY|
|Berry, Sue Ann - THE OHIO STATE UNIVERSITY|
|Hammond, Ronald - THE OHIO STATE UNIVERSITY|
|Madden, Laurence - THE OHIO STATE UNIVERSITY|
|Dorrance, Anne - THE OHIO STATE UNIVERSITY|
Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 1, 2010
Publication Date: February 1, 2010
Repository URL: http://hdl.handle.net/10113/41807
Citation: Redinbaugh, M.G., Molineros, J., Vacha, J., Berry, S., Hammond, R.B., Madden, L.V., Dorrance, A.E. 2010. Bean Pod Mottle Virus Spread in Insect Feeding Resistant Soybeans. Plant Disease. 94(2):265-270. Interpretive Summary: Bean pod mottle virus (BPMV) infections of soybeans reduce yield and seed quality across Ohio and the North Central U.S. While most viral diseases in crops are controlled using virus-resistant plants, no strong resistance to BPMV has been identified in soybeans. BPMV is spread in soybeans by the bean leaf beetle, and soybeans with resistance to bean leaf beetle feeding have been developed. We hypothesized that BPMV spread in the insect feeding resistant soybeans would be lower than in insect susceptible soybeans, and that the insect feeding resistance might be useful for soybean breeders trying to develop BPMV tolerant cultivars. We found that BPMV incidence was lower in two soybean cultivars with insect feeding resistance than in two susceptible cultivars. However, because the insect resistant lines were specialized, semi-dwarf lines, we used a similar insect susceptible line as a control. Interestingly, even lower BPMV incidence was found in this cultivar than in the two insect resistant cultivars. These results indicate that the insect resistance found in the soybeans we tested is not likely to be useful for developing BPMV tolerant cultivars.
Technical Abstract: Bean pod mottle virus (BPMV) reduces yield and seed quality in soybeans. No qualitative resistance to this virus has been found in soybean, although some tolerance is known. To test the hypothesis that virus incidence and movement would be reduced in soybeans with resistance to feeding by the virus' bean leaf beetle (Cerotoma trifurcata) vector, a split-plot experiment with four repetitions, five BPMV-susceptible host genotypes and two inoculum levels was carried out for two years at two Ohio locations. Host genotypes included the insect feeding susceptible cultivars Williams 82 and Resnik, insect feeding resistant, semi-dwarf cultivars HC95-15 and HC95-24, and the insect feeding susceptible, semi-dwarf cultivar Troll. BPMV incidence was assessed in individual plants on each of two sampling dates (at growth stages R2 and R7) using ELISA, and beetle feeding was visually assessed in 2004 BPMV incidence was assessed in individual plants at growth stages R2 and R7 using ELISA, and data were analyzed using a generalized linear mixed model. Within plots, BPMV incidence was highest in Resnik and Williams 82. BPMV incidence HC95-15 was not different than in Williams 82 and Resnik, but incidence in HC95-24 was lower than in Resnik and incidence in Troll was lower than in both Williams 82 and Resnik. BPMV incidence was also affected by year, location, inoculum level and sampling date. Beetle feeding was affected by location and genotype. Significant aggregation of BPMV infected plants was found for most plots, but aggregation was independent of host genotype and inoculum level. While the results indicate that BPMV infection varied by genotype, they do not support the hypothesis that insect feeding resistance would reduce the incidence and spread of BPMV.