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Title: Eight microsatellite loci in Phaedranassa schizantha Baker (Amaryllidaceae) and cross-amplification in other Phaedranassa species

Author
item OLEAS, NORA - FIU-DEPT OF BIO SCI
item Meerow, Alan
item FRANCISCO-ORTEGA, JAVIER - FIU-DEPT OF BIO SCI

Submitted to: Conservation Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2009
Publication Date: 6/1/2009
Citation: Oleas, N.H., Meerow, A.W., Francisco-Ortega, J. 2009. Eight microsatellite loci in Phaedranassa schizantha Baker (Amaryllidaceae) and cross-amplification in other Phaedranassa species. Conservation Genetics. DOI 10.1007/s10592-009-9846-8.

Interpretive Summary: We report on the isolation of eight DNA repeat markers from the tropical ornamental flower bulb Phaedranassa schizantha that will be used to analyze population structure and conservation status of this species and other related members of the genus.

Technical Abstract: Phaedranassa schizantha is a species endemic to Ecuador from which eight polymorphic microsatellite loci were isolated from an enriched genomic library. A total of 31 alleles with an average of four alleles per locus were detected across 29 individuals from a single natural population of P. schizantha. Observed heterozygosity ranged from 0.05 to 0.68. Most of the eight loci were successfully amplified in six Phaedranassa species. Five of those species are either “Vulnerable” or “Endangered” under IUCN criteria. These loci will be used to investigate patterns of inter- and intraspecific variation of Phaedranassa species, which will contribute data relevant to their conservation status.