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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Cryopreservation of orthodox and recalcitrant seed

Authors
item Normah, M. - UNIVERSITI MAYLAYSIA
item Choo, W. - UNIVERSITI MAYLAYSIA
item Kushnarenko, Svetlana - INSTITUTE OF PLANT BIO
item Reed, Barbara

Submitted to: Plant Tissue Culture and Development
Publication Type: Book / Chapter
Publication Acceptance Date: August 17, 2010
Publication Date: November 13, 2010
Citation: Normah, M.N., Choo, W.K., Kushnarenko, S.V., Reed, B.M. 2010. Cryopreservation of orthodox and recalcitrant seed. In: Trigiano, Robert N., Gray, Dennis J., editors. Plant Tissue Culture and Development. Boca Raton, Florida:Taylor & Francis (CRC Press, LCC). p. 507-514.

Interpretive Summary: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for storage of plant seeds in liquid nitrogen. It includes two exercises involving step by step protocols for use with the two major types of seeds. A standard technique for small seeds that can be dried (orthodox seed), and a technique for seed that can not be dried or frozen as whole seed (recalcitrant seed) are described in detail for use in undergraduate or graduate student class work.

Technical Abstract: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for cryopreservation of plant seeds in liquid nitrogen. It includes two exercises involving step by step protocols for use with the two major types of seeds. A standard technique for small seeds that can be dried (orthodox seed), and a technique for embryonic axes of seed that can not be dried or frozen as whole seed (recalcitrant seed) are described in detail for use in undergraduate or graduate student class work.

Last Modified: 9/10/2014
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