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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Cryopreservation of in vitro grown shoot tips

Authors
item Kushnarenko, Svetlana - INST PLANT BIO KAZAKHSTAN
item Reed, Barbara
item Normah, M. - UNIVERISTY OF MALAYSIA

Submitted to: Plant Tissue Culture and Development
Publication Type: Book / Chapter
Publication Acceptance Date: August 16, 2010
Publication Date: November 12, 2010
Citation: Kushnarenko, S.V., Reed, B.M., Normah, M.N. 2010. Cryopreservation of in vitro grown shoot tips. Trigiano, Robert N., Gray, Dennis J., editors. Plant Tissue Culture and Development and Biotechnology. Boca Raton, Florida: Taylor and Francis (CRS Press, LLC). p. 497-506.

Interpretive Summary: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for storage of the growing shoot tips of plants in liquid nitrogen. It includes two exercises involving step by step protocols for use with shoot tips. Vitrification (fast freezing) and encapsulation dehydration (drying) techniques are described in detail for use in undergraduate or graduate student class work.

Technical Abstract: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for cryopreservation of the growing shoot tips of plants in liquid nitrogen. It includes two exercises involving step by step protocols for use with shoot tips. Vitrification (fast freezing) and encapsulation dehydration techniques are described in detail for use in undergraduate or graduate student class work.

Last Modified: 9/2/2014
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