Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 12, 2009
Publication Date: January 12, 2009
Citation: Ulloa, M., Saski, C.A., Roberts, P.A. 2009. Genome regions' putative association with Fusarium wilt or root-knot nematode resistance in cotton. Plant and Animal Genome Conference.
Technical Abstract:
Around 1,300 microsatellite or SSR markers [named MUSB001 – MUSB1316 (600 informative)] were developed at the USDA-ARS, WICSRU Shafter, CA with the support of cooperators and Cotton Incorporated. These MUSB markers were developed from BAC-end DNA sequence information from a previously developed BAC library at the Clemson University Genomics Institute (CUGI) from DNA of the Acala Maxxa cultivar. A BAC clone corresponding to a diagnostic DNA marker can be used directly for map-based cloning of a gene or QTL when the marker is mapped to a locus closely linked to the gene or QTL of interest. From around 150 of these MUSB markers previously mapped and located on specific cotton chromosomes, we selected for sequencing 30 MUSB markers associated with or close to other markers that seem to be associated with Fusarium wilt (FOV) or root-knot nematode (RKN) resistance. The average BAC clone is around 140,000 bp. An average coverage from 5X to 7.5 X for each clone will be obtained using the shotgun approach. Progress in cotton genomics is lagging behind that in other agronomic crops. However, in the past decade it has seen substantial growth and development, conceptually and technically. It is important that we continue to obtain DNA sequence information from important chromosome regions for advancing marker development and gene discovery in cotton. This new DNA sequence can provide additional information to discover the genes responsible for plant defense mechanisms and resistance to FOV or RKN.
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