ARS | Publication request: 2008 FHB Analysis of Transgenic Barley Lines

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2008
Publication Date: December 2, 2008
Citation: Dahleen, L.S., Macky, R., Neate, S.M. 2008. 2008 FHB Analysis of Transgenic Barley Lines Meeting Proceedings of the National Fusarium Head Blight Forum. Indianapolis, IN; Dec 2-4, 2008 p.106

Technical Abstract: Transgenic lines have been developed with the goal of reducing FHB and DON in barley. Replicated field trials for FHB reaction of 48 Conlon transgenic lines were conducted in 2008 in Langdon, ND and Rosemount, MN. The Langdon trials consisted of three replicates in hill plots in an inoculated misted nursery and three replicates is the adjacent un-inoculated un-misted nursery. The misted nursery was inoculated with a mixture of five F. graminearum isolates three times at two weekly intervals beginning 2 weeks prior to heading. Rosemount plots included 4 replicates of 8 foot rows which were spray-inoculated at heading and misted after inoculation. FHB severity (% infected kernels) and FHB incidence (% infected spikes) were measured at both locations. Disease severity was highest in the Langdon inoculated plots (31-55%) and lowest in the plots at Rosemount (2-10%). FHB incidence at Langdon was essentially 100%, with almost all spikes showing some FHB except the resistant checks. Data were ranked from low to high for each measurement and Spearman rank correlations were not significant between the locations, i.e. lines with the lowest measurements in either Langdon nursery showed moderate to high FHB at Rosemount. Lines did show significant correlations between measurements within a location. Two lines showed significant reductions in FHB (compared to Conlon) in the Langdon un-inoculated nursery and two different lines showed significant reductions in incidence and severity at Rosemount. All four lines contained rice genes for a chitinase and a thaumatin-like protein. All 48 lines will be tested again in 2009 to validate the reductions in FHB.