ARS | Publication request: Cross-Species Amplification and Polymorphism of Microsatellite Loci in the Soybean Aphid, Aphis glycines

Submitted to: Journal of Economic Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 10, 2009
Publication Date: June 9, 2009
Repository URL: http://hdl.handle.net/10113/30448
Citation: Michel, A.P., Zhang, W., Jung, J., Kang, S., Mian, R.M. 2009. Cross-Species Amplification and Polymorphism of Microsatellite Loci in the Soybean Aphid, Aphis glycines. Journal of Economic Entomology. 102:1389-1392.

Interpretive Summary: Soybean aphid has become the most important insect pest of soybean in North America in recent years. Multiple biotypes of soybean aphids have been identified in USA. Molecular resources to differentiate among soybean aphid populations and biotypes are currently not available. We tested the utility of 18 previously characterized Aphis spp. microsatellite loci for polymorphism and differentiation among populations of soybean aphids, Aphis glycines. Loci were chosen from a closely related species (Aphis gossypii) and a more distantly related species (Aphis fabae). We found 11 loci to be polymorphic among Korean and North American populations. These loci will be valuable in characterizing population differentiation, biotypes, migration and adaptation of soybean aphid across geographic locations.

Technical Abstract: We tested the utility of 18 previously characterized Aphis spp. microsatellite loci for polymorphism and differentiation among populations of the soybean aphid, Aphis glycines. Loci were chosen from a closely related species (Aphis gossypii) and a more distantly related species (Aphis fabae). We found 11 loci to be polymorphic among Korean and North American populations. Overall expected heterozygosity was moderate (average, 0.48; range: 0-0.67). These loci will be valuable in characterizing population differentiation, migration and adaptation in an economically important pest of soybeans.