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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC CHARACTERIZATION AND IMPROVEMENT OF SHELLFISH BREEDING STOCKS FOR THE PACIFIC NORTHWEST Title: Nineteen Novel Microsatellite Markers for the Olympia Oyster, Ostrea conchaphila/lurida

Authors
item Stick, David - OSU
item Langdon, Chris - OSU
item Banks, Michael - OSU
item Camara, Mark

Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 19, 2008
Publication Date: January 31, 2009
Citation: Stick, D.A., Langdon, C.J., Banks, M.A., Camara, M.D. 2009. Nineteen Novel Microsatellite Markers for the Olympia Oyster, Ostrea conchaphila/lurida. Molecular Ecology Resources. 9: 153-155.

Interpretive Summary: In order to inform efforts aimed at restoring the native oyster, Ostrea conchaphila/lurida, in west coast estuaries, we need accurate information about population structure. This requires molecular markers that were not previously available. In this paper, we report 19 polymorphic microsatellites suitable for analyses of population differentiation, pedigree reconstruction and linkage map construction. In a Willapa Bay reference sample, mean observed and expected heterozygosities for these new microsatellite markers were 0.6729 and 0.8377. Nine loci deviated from Hardy-Weinberg equilibrium. These markers will be useful for a wide range of genetic studies of the Olympia oyster.

Technical Abstract: Accurate evaluation of remnant Ostrea conchaphila/lurida population structure is critical for developing appropriate restoration efforts. We report 19 polymorphic microsatellites suitable for analyses of population differentiation, pedigree reconstruction and linkage map construction. We screened clones from four enriched genomic libraries, identified 73 microsatellite-containing sequences and designed PCR primers for 44 sequences. We successfully optimized PCR conditions for 20 loci, including one monomorphic locus. In a Willapa Bay reference sample, mean HO and HE were 0.6729 and 0.8377. Nine loci deviated from Hardy-Weinberg equilibrium. These markers have proven useful for genetic studies of the Olympia oyster.

Last Modified: 10/23/2014
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